Molecular and cytological analysis of the expression of Streptomyces sporulation regulatory gene whiH.
Författare
Summary, in English
The whiH gene is required for the orderly sporulation septation that divides aerial hyphae into spores in Streptomyces coelicolor. Here, we use a whiHp-mCherry transcriptional reporter construct to show that whiHp is active specifically in aerial hyphae, fluorescence being dependent on sporulation sigma factor WhiG. The results show that the promoter is active before the septation event that separates the sub-apical compartment from the tip compartment destined to become a spore chain. We conclude that WhiG-directed RNA polymerase activity, which is required for whiH transcription, must precede this septation event and is not restricted to apical sporogenic compartment of the aerial hyphae. Further, it is demonstrated that WhiH, a predicted member of the GntR family of transcription factors, is able to bind specifically to a sequence in its own promoter, strongly suggesting that it acts as an autoregulatory transcription factor. Finally, we show by site-directed mutagenesis and a genetic complementation test that whiH is translated from a start codon overlapping with the previously identified transcription start point, implying leaderless transcription.
Avdelning/ar
Publiceringsår
2013
Språk
Engelska
Sidor
96-105
Publikation/Tidskrift/Serie
FEMS Microbiology Letters
Volym
341
Issue
2
Dokumenttyp
Artikel i tidskrift
Förlag
Oxford University Press
Ämne
- Biological Sciences
Nyckelord
- GntR family
- whiG
- FtsZ
- differentiation
- developmental regulation
- cell division
Status
Published
Forskningsgrupp
- Microbiology Group
ISBN/ISSN/Övrigt
- ISSN: 1574-6968