Structure of muramic acid TMS derivative mass spectrum's base ion (m/z=185) used for quantification of bacterial peptidoglycan.

Summary, in English

The use of trimethylsilyl (TMS)-derivatisation for determining muramic acid in environmental and clinical samples by gas chromatography-mass spectrometry provides high detection sensitivity; however, questions have been raised as concerns the chemical structure of the entity giving the strong signal of m/z 185. In the present communication we present evidence that this entity results from the formation of a lactam structure of muramic acid upon derivatisation.

• Författare: Karol Bal; Lennart Larsson; Eugenia Mielniczuk; Zbigniew Mielniczuk
• Avdelning/ar: Avdelningen för medicinsk mikrobiologi
• Publiceringsår: 2002
• Språk: Engelska
• Sidor: 267-270
• Publikation/Tidskrift/Serie: Journal of Microbiological Methods
• Volym: 48
• Issue: 2-3
• Länkar: http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11777574&dopt=Abstract; http://dx.doi.org/10.1016/S0167-7012(01)00328-1
• Dokumenttyp: Artikel i tidskrift
• Förlag: Elsevier
• Ämne: Microbiology in the medical area
• Nyckelord: Muramic Acids : chemistry; Trimethylsilyl Compounds : chemistry; Spectrum Analysis Mass; Peptidoglycan : analysis; Bacteria : chemistry
• Status: Published
• ISBN/ISSN/Övrigt: ISSN: 1872-8359