Purification of a rotenone-insensitive NAD(P)H dehydrogenase from the inner surface of the inner membrane of red beetroot mitochondria

Summary, in English

The soluble fraction of disrupted red beetroot mitochondria was resolved by anion-exchange chromatography. Three NADH-oxidising activities were found, including one duroquinone reductase oxidising both NADH and NADPH. This NAD(P)H-duroquinone reductase, which we assign as the internal rotenone-insensitive NAD(P)H dehydrogenase, was further purified by affinity chromatography into a 26 kDa polypeptide.

• Författare: Allan G. Rasmusson; Kenneth M. Fredlund; Ian M. Møller
• Avdelning/ar: Molekylär cellbiologi; Växtbiologi
• Publiceringsår: 1993-02-08
• Språk: Engelska
• Sidor: 107-110
• Publikation/Tidskrift/Serie: Biochimica et Biophysica Acta - Bioenergetics
• Volym: 1141
• Issue: 1
• Länkar: http://dx.doi.org/10.1016/0005-2728(93)90196-M
• Dokumenttyp: Artikel i tidskrift
• Förlag: Elsevier
• Ämne: Biochemistry and Molecular Biology
• Nyckelord: (Plant); Enzyme purification; Inner membrane; Mitochondrion; NAD(P)H dehydrogenase; Protein purification; Respiratory chain; Rotenone insensitivity
• Status: Published
• Forskningsgrupp: Plant Biology
• ISBN/ISSN/Övrigt: ISSN: 0005-2728