Design of recombinant antibody microarrays for membrane protein profiling of cell lysates and tissue extracts.
Författare
Summary, in English
Generating global protein expression profiles, including also membrane proteins, will be crucial for our understanding of biological processes in health and disease. In this study, we have expanded our antibody microarray technology platform and designed the first human recombinant antibody microarray for membrane proteins targeting crude cell lysates and tissue extracts. We have optimized all key technological parameters and successfully developed a setup for extracting, labeling and analyzing non-fractionated membrane proteomes under non-denaturing conditions. Finally, the platform was also extended and shown to be compatible with simultaneous profiling of both membrane proteins and water-soluble proteins.
Avdelning/ar
- BioCARE: Biomarkers in Cancer Medicine improving Health Care, Education and Innovation
- Institutionen för immunteknologi
- Obstetrik och gynekologi, Lund
Publiceringsår
2011
Språk
Engelska
Sidor
1550-1554
Publikation/Tidskrift/Serie
Proteomics
Volym
11
Dokumenttyp
Artikel i tidskrift
Förlag
John Wiley & Sons Inc.
Ämne
- Obstetrics, Gynecology and Reproductive Medicine
Status
Published
ISBN/ISSN/Övrigt
- ISSN: 1615-9861