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Silicon microstructures for high-speed and high-sensitivity protein identifications

Författare:
Publiceringsår: 2001
Språk: Engelska
Sidor: 217-232
Publikation/Tidskrift/Serie: Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
Volym: 752
Nummer: 2
Dokumenttyp: Artikel i tidskrift
Förlag: Elsevier

Sammanfattning

Silicon microtechnology has been used to develop a microstructure toolbox in order to enable high accuracy protein identification. During the last 2 years we developed and applied monocrystalline silicon structures and established new automated protein analysis platforms, The development of a high throughput protein platform is presented where fully automated protein identifications are performed. It includes the reduction and alkylation of the protein sample in a standard 96- or 384-well plate format prior to injection of 1 mul samples into the continuous flow based microtechnology platform. The processed sample is transferred to a microchip nanovial array target using piezoelectric microdispensing. identification is made by MALDI-TOF MS and a database search. After the initial sample reduction and alkylation period of 50 min the platform can digest and process protein samples at a speed of 100 samples in 210 min. An optional configuration of the platform, operating the dispenser in the 'static mode', enables on-target enrichment of low abundant proteins and peptides e.g. from 2DE samples. This makes detection at the low attomole level possible. (C) 2001 Elsevier Science B.V. All rights reserved.

Nyckelord

  • Medical Engineering
  • silicon microstructures
  • proteins
  • FLIGHT MASS-SPECTROMETRY
  • FLOW-THROUGH DISPENSER
  • SAMPLE PREPARATION
  • POROUS SILICON
  • MATRIX
  • MALDI

Övriga

Published
  • ISSN: 0378-4347

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