Effects of long-term portal hypertension on structure, active force and content of contractile and structural proteins in smooth muscle of the rat portal vein
Författare
Summary, in English
Growth of the smooth muscle cells in the rat portal vein was induced by a partial ligation of the vessel. The ligation caused an increase in the transmural pressure and segments of the portal vein were investigated 6 weeks after the ligation. The spontaneous contractile activity of the ligated veins was similar to that of the control veins. In the ligated vessels the active force at optimal length for force development was doubled, 22.8 +/- 1.3 compared with 12.5 +/- 1.4 mN for the controls. The cross-sectional area of the media in the ligated veins, determined on transverse sections, increased from the control value of 0.10 +/- 0.01 to 0.19 +/- 0.01 mm2. Electron microscopy revealed that the mean cross-sectional area of the smooth muscle cells in the ligated portal vein was doubled (controls: 6.4 +/- 0.6, hypertrophic: 13.6 +/- 1.8 microns2). This suggests hypertrophy of the smooth muscle cells in the vessel wall as the cause for the increase in cross-sectional area of the ligated veins. An increase in the number of intermediate filaments was observed in the hypertrophied smooth muscle. The relative contents of contractile (myosin and actin) and structural (desmin and vimentin) proteins were determined with SDS-polyacrylamide gel electrophoresis. The actin/myosin and vimentin/actin ratios were unaltered by hypertrophy. The hypertrophied veins showed an increase in the desmin/actin ratio (control: 0.20 +/- 0.01, hypertrophied: 0.27 +/- 0.03). The increased amounts of desmin correlates with the increased number of intermediate filaments observed by electron microscopy.(ABSTRACT TRUNCATED AT 250 WORDS)
Avdelning/ar
Publiceringsår
1994
Språk
Engelska
Sidor
171-179
Publikation/Tidskrift/Serie
Acta Physiologica Scandinavica
Volym
150
Issue
2
Dokumenttyp
Artikel i tidskrift
Förlag
Wiley-Blackwell
Ämne
- Pharmacology and Toxicology
- Medicinal Chemistry
Status
Published
ISBN/ISSN/Övrigt
- ISSN: 0001-6772