Cell-surface charge and cell-surface hydrophobicity of collagen-binding Aeromonas and Vibrio strains
Författare
Summary, in English
Abstract Partitioning in aqueous polymer two-phase systems of polyethylene glycol and dextran was used to detect and compare cell-surface charge and cell-surface hydrophobicity of Aeromonas hydrophila, A. caviae, A. sobria, Vibrio cholerae, and V. anguillarum strains. These strains have cell-surface components that bound either native or thermally denatured type I collagen (i.e., a mixture of the alpha1+alpha2 chains) and gelatin immobilized on latex beads. Our goals were: (1) to compare the possible relationship between the cell-surface charge/hydrophobicity and binding to collagen and (2) to evaluate the influence of the culture media on the expression of surface properties. There was no apparent relationship between cell-surface charge, cell-surface hydrophobicity, and binding to collagen. The expression of surface properties was dependent on the culture media. There was no relationship between binding to immobilized collagen and binding to soluble 125I-labeled collagen. Particle-agglutination reactivity differed when using various collagen-coated microbead preparations. There were general differences in the particle-agglutination reactivity when collagen-coated latex beads were prepared using different coating procedures. The negative charge and hydrophobicity of the various collagen-coated microbead preparations were also studied by partitioning in the two-phase system of polyethylene glycol and dextran. Under these conditions, the alpha1+alpha2 collagen-chain mixture covalently immobilized on carboxy-modified latex beads was less hydrophobic and negatively charged than gelatin and native collagen immobilized on the same kind of latex beads. For latex beads passively coated with collagen preparations, the alpha1+alpha2 collagen-chain mixture was more hydrophobic than gelatin and native collagen. We suggest that for screening collagen-binding among Vibrio and Aeromonas strains, a reliable and sensitive particle-agglutination assay should consider the collagen preparation and the coating procedure for the immobilization of collagen onto the latex beads. In this regard, carboxy-modified latex beads coated with an alpha1+alpha2 collagen-chain mixture gave the best results.
Avdelning/ar
Publiceringsår
1995
Språk
Engelska
Sidor
223-230
Publikation/Tidskrift/Serie
Archives of Microbiology
Volym
164
Issue
3
Dokumenttyp
Artikel i tidskrift
Förlag
Springer
Ämne
- Microbiology in the medical area
Nyckelord
- Key words Cell-surface hydrophobicity . Cell-surface charge . Collagen . Aeromonas caviae . Aeromonas . hydrophila . Aeromonas sobria . Vibrio anguillarum . Vibrio cholerae
Status
Published
ISBN/ISSN/Övrigt
- ISSN: 0302-8933