Analysis of vesicular trafficking in primary neurons by live imaging.

Summary, in English

Alzheimer's disease, the most common neurodegenerative disease, is characterized by a progressive loss of synapses and accumulation of amyloid-beta (Aβ) peptides in the brain. Previous studies demonstrated that acute increase in synaptic activity in cultured hippocampal slices and mouse brains (Cirrito et al. Neuron 48: 913-922, 2005; Kamenetz et al. Neuron 37: 925-937, 2003) enhanced secretion of Aβ. Since synaptic activity promotes Aβ secretion, it could also affect the trafficking and processing of its precursor, the amyloid precursor protein (APP). Here, we describe a method to investigate the effect of acute synaptic activation on APP trafficking within dendrites.

• Författare: Davide Tampellini; Gunnar Gouras
• Avdelning/ar: Experimentell demensforskning; MultiPark: Multidisciplinary research focused on Parkinson´s disease
• Publiceringsår: 2011
• Språk: Engelska
• Sidor: 343-350
• Publikation/Tidskrift/Serie: Methods in Molecular Biology
• Volym: 793
• Länkar: http://www.ncbi.nlm.nih.gov/pubmed/21913111?dopt=Abstract; http://dx.doi.org/10.1007/978-1-61779-328-8_22
• Dokumenttyp: Artikel i tidskrift
• Förlag: Springer
• Ämne: Neurosciences
• Status: Published
• Forskningsgrupp: Experimental Dementia Research
• ISBN/ISSN/Övrigt: ISSN: 1940-6029