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Feeding strategies for E-coli fermentations demanding an enriched environment

Författare:
Publiceringsår: 2007
Språk: Engelska
Sidor: 13-25
Publikation/Tidskrift/Serie: BIOPROCESS AND BIOSYSTEMS ENGINEERING
Volym: 30
Nummer: 1
Dokumenttyp: Artikel
Förlag: SPRINGER

Sammanfattning

The addition of a carbon nutrient feed to a fed-batch cultivation is often not enough to obtain satisfactory growth and/or production. In some cases, an additional feed with for example supplementary amino acids or complex media is required. This work presents the development of feeding strategies where more than one feed is required and the knowledge of the growth requirements is low. Simulations and cultivations with E. coli are shown using the proposed feed controllers which are based on a probing control concept. The strategies work well and they can be used to shorten the process development phase considerably.

Disputation

Nyckelord

  • Technology and Engineering
  • Escherichia coli
  • complex medium
  • amino acid
  • fed-batch cultivation
  • fermentation process
  • feeding strategy

Övriga

Published
Yes
  • ISSN: 1615-7591

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