The cysteine protease inhibitors cystatins inhibit herpes simplex virus type 1-induced apoptosis and virus yield in HEp-2 cells
Författare
Summary, in English
The role of cystatins in herpes simplex virus (HSV)-induced apoptosis and viral replication has been studied. Human epithelial (HEp-2) cells infected with wild-type HSV-1 (F), with a deletion virus lacking the anti-apoptotic gene Us3 (R7041) or with a deletion virus lacking the anti-apoptotic genes Us3 and ICP4 (d120) were treated with cystatin A, C or D. Cells and culture media were studied at different time points for replicating HSV-1 and for apoptosis. Cystatins C and D inhibited the yield of replicative HSV-1 significantly in HEp-2 cells. In addition, cystatin D inhibited R7041 and d120 virus-induced apoptosis. Moreover, cystatin A inhibited R7041 induced apoptosis. These inhibitory effects of cystatins on virus replication and apoptosis are likely to be separate functions. Cystatin D treatment decreased cellular cathepsin B activity in HSV-1 infection, suggesting that cathepsin B is involved in virus-induced apoptosis.
Avdelning/ar
Publiceringsår
2007
Språk
Engelska
Sidor
2101-2105
Publikation/Tidskrift/Serie
Journal of General Virology
Volym
88
Issue
8
Dokumenttyp
Artikel i tidskrift
Förlag
Microbiology Society
Ämne
- Pharmacology and Toxicology
- Medicinal Chemistry
Status
Published
ISBN/ISSN/Övrigt
- ISSN: 1465-2099