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An update and optimisation of oligonucleotide probes targeting methanogenic Archaea for use in fluorescence in situ hybridisation (FISH)

In English

Författare

Summary, in English

Fluorescence in situ hybridisation (FISH) is a common and popular method used to investigate microbial populations in natural and engineered environments. DNA oligonucleoticle probes require accurate determination of the optimal experimental conditions for their use in FISH Oligonucleotides targeting the rRNA of methanogenic Archaea at various taxonomic levels have previously been published, although when applied in FISH, no optimisation data has been presented In this study, 3000 Euryarchaeota 16S rRNA gene sequences were phylogenetically analysed and previously published oligonucleoticles were evaluated for target group accuracy. Where necessary, modifications were introduced or new probes were designed. The updated set of probes was optimised for use in FISH for a more accurate detection of methanogenic Archaea. (c) 2005 Elsevier B.V. All rights reserved.

Publiceringsår

2006

Språk

Engelska

Sidor

194-201

Publikation/Tidskrift/Serie

Journal of Microbiological Methods

Volym

65

Issue

1

Dokumenttyp

Artikel i tidskrift

Förlag

Elsevier

Ämne

  • Industrial Biotechnology

Nyckelord

  • Euryarchaeota
  • oligonucleotide
  • 16S rDNA
  • mnethanogen
  • FISH

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 1872-8359