Effect of hindered diffusion on the adsorption of proteins in agarose gel using a pore model
Författare
Summary, in English
The hindered diffusion and binding of proteins of different sizes (lysozyme, BSA and IgG) in an agarose gel is described using adsorption kinetic and diffusional data together with an experimentally determined pore size distribution in the gel. The validity of the pore model, including variable diffusion coefficients and porosities is tested against experimental confocal microscopy data. No fitting parameters were used in the present model. The importance of knowing the gel structure is demonstrated especially for large proteins such as IgG. Experimental confocal microscopy data can be explained by the present model. (C) 2004 Elsevier B.V. All rights reserved.
Avdelning/ar
Publiceringsår
2004
Språk
Engelska
Sidor
161-172
Publikation/Tidskrift/Serie
Journal of Chromatography A
Volym
1048
Issue
2
Dokumenttyp
Artikel i tidskrift
Förlag
Elsevier
Ämne
- Chemical Engineering
Status
Published
ISBN/ISSN/Övrigt
- ISSN: 0021-9673