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Two different dihydroorotate dehydrogenases from yeast Saccharomyces kluyveri

In English

Författare

  • Elke Zameitat
  • W. Knecht
  • Jure Piskur
  • M. Löffler

Summary, in English

Genes for two structurally and functionally different dihydroorotate dehydrogenases (DHODHs, EC 1.3.99.11), catalyzing the fourth step of pyrimidine biosynthesis, have been previously found in yeast Saccharomyces kluyveri. One is closely related to the Schizosaccharomyces pombe mitochondrial family 2 enzymes, which use quinones as direct and oxygen as the final electron acceptor. The other one resembles the Saccharomyces cerevisiae cytosolic family 1A fumarate-utilizing DHODH. The DHODHs from S. kluyveri, Sch. pombe and S. cerevisiae, were expressed in Escherichia coli and compared for their biochemical properties and interaction with inhibitors. Benzoates as pyrimidine ring analogs were shown to be selective inhibitors of cytosolic DHODs. This unique property of Saccharomyces DHODHs could appoint DHODH as a species-specific target for novel anti-fungal therapeutics

Publiceringsår

2004

Språk

Engelska

Sidor

129-134

Publikation/Tidskrift/Serie

FEBS Letters

Volym

568

Issue

1-3

Dokumenttyp

Artikel i tidskrift

Förlag

Wiley-Blackwell

Ämne

  • Biological Sciences

Nyckelord

  • Dihydroorotate dehydrogenase
  • Schizosaccharomyces pombe
  • Saccharomyces kluyveri
  • Saccharomyces cerevisiae
  • nucleic acid precursor
  • evolution
  • yeast
  • enzyme
  • Protein expression
  • Inhibition

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 1873-3468