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Robotic implementation of a microchip-based protein clean-up and enrichment system for MALDI-TOF MS readout

Författare:
Publiceringsår: 2006
Språk: Engelska
Sidor: 3147-3153
Publikation/Tidskrift/Serie: Measurement Science and Technology
Volym: 17
Nummer: 12
Dokumenttyp: Artikel

Sammanfattning

A capillary filling microfluidic proteomic sample processing system has been realized in an automated set-up. Chip-integrated solid-phase extraction is performed in a 96-array format followed by sequential capillary action elution into a piezoelectric microdispenser and subsequent transfer to MALDI targets. Samples are eluted and deposited in volumes of 200–300 nl. The robotic system offers calibration to user-defined microextraction arrays and MALDI-target formats at micrometre resolution. Built-in force feedback control ensures a precise and robust microchip docking/handling in three dimensions. An efficient automated washing protocol eliminates analyte carry-over. System throughput ranges typically from 50–100 samples h-1.

Disputation

Nyckelord

  • Chemistry
  • Technology and Engineering
  • piezodispensing
  • capillary force
  • microfluidics
  • MALDI
  • mass spectrometry
  • microchip
  • robotic
  • MALDI-TOF MS
  • automation
  • protein analysis
  • proteomics
  • solid phase extraction

Övriga

Published
Yes
  • ISSN: 0957-0233

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