Webbläsaren som du använder stöds inte av denna webbplats. Alla versioner av Internet Explorer stöds inte längre, av oss eller Microsoft (läs mer här: * https://www.microsoft.com/en-us/microsoft-365/windows/end-of-ie-support).

Var god och använd en modern webbläsare för att ta del av denna webbplats, som t.ex. nyaste versioner av Edge, Chrome, Firefox eller Safari osv.

Robotic implementation of a microchip-based protein clean-up and enrichment system for MALDI-TOF MS readout

Författare

Summary, in English

A capillary filling microfluidic proteomic sample processing system has been realized in an automated set-up. Chip-integrated solid-phase extraction is performed in a 96-array format followed by sequential capillary action elution into a piezoelectric microdispenser and subsequent transfer to MALDI targets. Samples are eluted and deposited in volumes of 200–300 nl. The robotic system offers calibration to user-defined microextraction arrays and MALDI-target formats at micrometre resolution. Built-in force feedback control ensures a precise and robust microchip docking/handling in three dimensions. An efficient automated washing protocol eliminates analyte carry-over. System throughput ranges typically from 50–100 samples h-1.

Publiceringsår

2006

Språk

Engelska

Sidor

3147-3153

Publikation/Tidskrift/Serie

Measurement Science & Technology

Volym

17

Issue

12

Dokumenttyp

Artikel i tidskrift

Förlag

IOP Publishing

Ämne

  • Analytical Chemistry
  • Medical Engineering

Nyckelord

  • piezodispensing
  • capillary force
  • microfluidics
  • MALDI
  • mass spectrometry
  • microchip
  • robotic
  • MALDI-TOF MS
  • automation
  • protein analysis
  • proteomics
  • solid phase extraction

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 0957-0233