Robotic implementation of a microchip-based protein clean-up and enrichment system for MALDI-TOF MS readout
Författare
Summary, in English
A capillary filling microfluidic proteomic sample processing system has been realized in an automated set-up. Chip-integrated solid-phase extraction is performed in a 96-array format followed by sequential capillary action elution into a piezoelectric microdispenser and subsequent transfer to MALDI targets. Samples are eluted and deposited in volumes of 200–300 nl. The robotic system offers calibration to user-defined microextraction arrays and MALDI-target formats at micrometre resolution. Built-in force feedback control ensures a precise and robust microchip docking/handling in three dimensions. An efficient automated washing protocol eliminates analyte carry-over. System throughput ranges typically from 50–100 samples h-1.
Avdelning/ar
Publiceringsår
2006
Språk
Engelska
Sidor
3147-3153
Publikation/Tidskrift/Serie
Measurement Science & Technology
Volym
17
Issue
12
Dokumenttyp
Artikel i tidskrift
Förlag
IOP Publishing
Ämne
- Analytical Chemistry
- Medical Engineering
Nyckelord
- piezodispensing
- capillary force
- microfluidics
- MALDI
- mass spectrometry
- microchip
- robotic
- MALDI-TOF MS
- automation
- protein analysis
- proteomics
- solid phase extraction
Status
Published
ISBN/ISSN/Övrigt
- ISSN: 0957-0233