Molecular cloning of epididymal and seminal vesicular transcripts encoding a semenogelin-related protein
Författare
Summary, in English
Freshly ejaculated human semen has the appearance of a loose gel in which the predominant structural protein components are the seminal vesicle-secreted semenogelins (Sg). The primary structure of the 439-residue SgI has previously been obtained by cDNA cloning. This cDNA cross-hybridizes to a larger transcript coding for a second secretory protein, SgII. Here we report the almost complete structure of a precursor of SgII established by lambda gt11 clones isolated from epididymal and seminal vesicular cDNA libraries. The deduced amino acid sequence of the 559-residue mature protein has a molecular weight of 62,931 but an increase in weight may be provided by asparagine-linked oligosaccharide attachment at residue 249. SgII, which has 78% overall identity with SgI, contains eight 60-residue regions that display conspicuous internal sequence similarity, whereas SgI only contains six of these regions. The SgII structure is translated from an open reading frame in a polyadenylylated 2.4-kilobase transcript. The message is abundant in the seminal vesicles but rare in the epididymis.
Avdelning/ar
Publiceringsår
1992
Språk
Engelska
Sidor
63-4559
Publikation/Tidskrift/Serie
Proc Natl Acad Sci U S A
Volym
89
Issue
10
Länkar
Dokumenttyp
Artikel i tidskrift
Ämne
- Medicinal Chemistry
Nyckelord
- Research Support
- Protein Precursors/*genetics
- Oligodeoxyribonucleotides
- Molecular Weight
- Molecular Sequence Data
- Male
- Humans
- Gonadal Steroid Hormones/*genetics
- Gene Library
- Epididymis/*physiology
- DNA/genetics/isolation & purification
- Comparative Study
- Molecular/methods
- Cloning
- Northern
- Blotting
- Amino Acid Sequence
- Base Sequence
- Non-U.S. Gov't
- Restriction Mapping
- Semen/*physiology
- *Seminal Plasma Proteins
- *Seminal Vesicle Secretory Proteins
- Seminal Vesicles/*physiology
- Sequence Homology
- Nucleic Acid
- *Transcription
- Genetic
Status
Published
Forskningsgrupp
- Clinical Chemistry, Malmö