Chlorhexidine for prevention of neonatal colonization with group B streptococci. I. In vitro effect of chlorhexidine on group B streptococci
Författare
Summary, in English
Forty-three strains of group B streptococci (GBS) of types Ia, Ib, II and III were tested for susceptibility to chlorhexidine in concentrations ranging from 256 to 0.25 mg/l using the agar and tube dilution methods. The strains showed minimum inhibitory concentration (MIC) values ranging from 0.5 to 1 mg/l. Serum added to the test medium (50%) increased the MIC values to 4-8 mg/l, while amniotic fluid (50%) had almost no effect, increasing the values to 1-2 mg/l. The minimum bactericidal concentration (MBC) ranged from 1 to 5 mg/l. The killing kinetics were related to the concentration of chlorhexidine and the length of exposure. For example, at a concentration of 63 mg/l, 7 h were required for a bactericidal effect in broth, as compared to 1 h at 500 mg/l chlorhexidine. 200 mg/l chlordexidine had no effect on the adherence of two GBS strains to vaginal epithelial cells, and no effect on the phagocytosis of GBS with mouse peritoneal macrophages.
Avdelning/ar
Publiceringsår
1983
Språk
Engelska
Sidor
157-165
Publikation/Tidskrift/Serie
European Journal of Obstetrics, Gynecology, and Reproductive Biology
Volym
16
Issue
3
Dokumenttyp
Artikel i tidskrift
Förlag
Elsevier
Ämne
- Obstetrics, Gynecology and Reproductive Medicine
Nyckelord
- group B streptococci
- chlorhexidine
- minimal inhibitory concentration
- minimal bactericidal concentration
- adherence
- phagocytosis
Status
Published
ISBN/ISSN/Övrigt
- ISSN: 0301-2115