Sensitivity of HIV-1 primary isolates to human anti-CD40 antibody-mediated suppression is related to co-receptor use
Författare
Summary, in English
The effect of CD40 ligation on infection by HIV-1 primary isolates with different R5 phenotypes was evaluated with a novel set of anti-CD40 monoclonal antibodies originating from a human phage display library. Five human monoclonal anti-CD40 antibodies of IgG1 subtype characterized by the ability to activate B cells via CD40 were tested for induction of the CC-chemokines RANTES and MIP-1alpha and inhibition of HIV-1 replication in primary monocyte-derived macrophages (MDM). All activating anti-CD40 antibodies were able to induce CC-chemokines in MDM. We chose the most potent antibody, clone B44, for further experiments. This antibody had a suppressive effect on HIV-1 isolates of the R5 phenotype with limited use of CCR5/CXCR4 chimeric receptors. In comparison, HIV-1 isolates with broader use of CCR5/CXCR4 chimeric receptors or with CXCR4 use were less sensitive to anti-CD40-induced suppression. The results indicate that HIV-1 replication is inhibited by human anti-CD40 monoclonal antibodies through the mechanism of CC-chemokine induction. This effect is thus restricted to HIV-1 isolates sensitive to inhibition by CC-chemokines.
Avdelning/ar
- Institutionen för immunteknologi
- Drug Target Discovery
- Avdelningen för medicinsk mikrobiologi
Publiceringsår
2008
Språk
Engelska
Sidor
447-452
Publikation/Tidskrift/Serie
AIDS Research and Human Retroviruses
Volym
24
Issue
3
Dokumenttyp
Artikel i tidskrift
Förlag
Mary Ann Liebert, Inc.
Ämne
- Pharmacology and Toxicology
- Microbiology in the medical area
Status
Published
Forskningsgrupp
- Drug Target Discovery
ISBN/ISSN/Övrigt
- ISSN: 1931-8405