NT-proBNP on Cobas h 232 in point-of-care testing: Performance in the primary health care versus in the hospital laboratory
Författare
Summary, in English
Background. NT-proBNP may be useful for ruling out heart failure in primary health care. In this study we examined the analytical quality of NT-proBNP in primary health care on the Cobas h 232 point-of-care instrument compared with measurements performed in a hospital laboratory. Materials and methods. Blood samples requested for NT-proBNP were collected in primary health care (n = 95) and in a hospital laboratory (n = 107). NT-proBNP was measured on-site on Cobas h 232 instruments both in primary health care centres and at the hospital laboratory and all samples were also analyzed with a comparison method at the hospital. Precision, trueness, accuracy, and lot-variation were determined at different concentration levels and evaluated according to acceptance criteria. Furthermore user-friendliness was assessed by questionnaires. Results. For Cobas h 232 repeatability CV was 8.5-10.7% in the hospital setting and 5.3-10.0% in the primary health care and within the analytical quality specifications, but higher than with the comparison method (<4%). NT-proBNP results obtained in primary health care were significantly higher than by the hospital comparison method (bias ranged from 14.3-23.7%), whereas there was no significant bias when Cobas h 232 was used in the hospital setting (bias ranged from 4.9 to 7.0%). User-friendliness of Cobas h 232 was overall acceptable. Conclusion. Cobas h 232 point-of-care instrument for measurement of NT-proBNP performed satisfactorily with regard to precision, user-friendliness, and lot-variation. A decrease in NT-proBNP levels observed in samples transported to a central laboratory needs further attention and investigation.
Avdelning/ar
Publiceringsår
2015
Språk
Engelska
Sidor
602-609
Publikation/Tidskrift/Serie
Scandinavian Journal of Clinical & Laboratory Investigation
Volym
75
Issue
7
Dokumenttyp
Artikel i tidskrift
Förlag
Informa Healthcare
Ämne
- Health Care Service and Management, Health Policy and Services and Health Economy
- Cardiac and Cardiovascular Systems
Nyckelord
- Natriuretic peptide
- biological markers
- heart disease
- point-of-care
- systems
- validation studies
Status
Published
ISBN/ISSN/Övrigt
- ISSN: 1502-7686