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Deoxyribonucleoside kinases in two aquatic bacteria with high specificity for thymidine and deoxyadenosine.

In English

Författare

  • Tinkara Tinta
  • Louise Slot Christiansen
  • Anke Konrad
  • David A Liberles
  • Valentina Turk
  • Birgitte Munch-Petersen
  • Jure Piskur
  • Anders Ranegaard Clausen

Summary, in English

Deoxyribonucleoside kinases (dNKs) are essential in the mammalian cell but their 'importance' in bacteria, especially aquatic ones, is less clear. We studied two aquatic bacteria, Gram-negative Flavobacterium psychrophilum JIP02/86 and Polaribacter sp. MED152, for their ability to salvage deoxyribonucleosides (dNs). Both had a Gram-positive-type thymidine kinase (TK1), which could phosphorylate thymidine, and one non-TK1 dNK, which could efficiently phosphorylate deoxyadenosine and slightly also deoxycytosine. Surprisingly, the four tested dNKs could not phosphorylate deoxyguanosine, and apparently, these two bacteria are missing this activity. When tens of available aquatic bacteria genomes were examined for the presence of dNKs, a majority had at least a TK1-like gene, but several lacked any dNKs. Apparently, among aquatic bacteria, the role of the dN salvage varies.

Publiceringsår

2012

Språk

Engelska

Sidor

120-127

Publikation/Tidskrift/Serie

FEMS Microbiology Letters

Volym

331

Issue

2

Dokumenttyp

Artikel i tidskrift

Förlag

Oxford University Press

Ämne

  • Biological Sciences

Nyckelord

  • aquatic bacteria
  • 3H-thymidine incorporation
  • deoxyribonucleoside kinase
  • nucleoside salvage

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 1574-6968