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A role for PKC-epsilon in Fc gamma R-mediated phagocytosis by RAW 264.7 cells

In English

Författare

  • EC Larsen
  • T Ueyama
  • PM Brannock
  • Y Shirai
  • N Saito
  • Christer Larsson
  • D Loegering
  • PB Weber
  • MR Lennartz

Summary, in English

Protein kinase C (PKC) plays a prominent role in immune signaling, and the paradigms for isoform selective signaling are beginning to be elucidated. Real-time microscopy was combined with molecular and biochemical approaches to demonstrate a role for PKC-epsilon in Fcgamma receptor (FcgammaR)-dependent phagocytosis. RAW 264.7 macrophages were transfected with GFP-conjugated PKC isoforms, and GFP movement was followed during phagocytosis of fluorescent IgG-opsonized beads. PKC-epsilon, but not PKC-delta, concentrated around the beads. PKC-epsilon accumulation was transient; apparent as a "flash" on target ingestion. Similarly, endogenous PKC-epsilon was specifically recruited to the nascent phagosomes in a time-dependent manner. Overexpression of PKC-epsilon, but not PKC-alpha, PKC-delta, or PKC-gamma enhanced bead uptake 1.8-fold. Additionally, the rate of phagocytosis in GFP PKC-epsilon expressors was twice that of cells expressing GFP PKC-delta. Expression of the regulatory domain (ERD) and the first variable region (epsilonV1) of PKC-epsilon inhibited uptake, whereas the corresponding PKC-delta region had no effect. Actin polymerization was enhanced on expression of GFP PKC-epsilon and ERD, but decreased in cells expressing epsilonV1, suggesting that the epsilonRD and epsilonV1 inhibition of phagocytosis is not due to effects on actin polymerization. These results demonstrate a role for PKC-epsilon in FcgammaR-mediated phagocytosis that is independent of its effects on actin assembly.

Publiceringsår

2002

Språk

Engelska

Sidor

939-944

Publikation/Tidskrift/Serie

Journal of Cell Biology

Volym

159

Issue

6

Dokumenttyp

Artikel i tidskrift

Förlag

Rockefeller University Press

Ämne

  • Cancer and Oncology

Nyckelord

  • immunoglobulin
  • protein kinase C-epsilon
  • signal transduction
  • macrophage
  • confocal

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 0021-9525