RNAi screen identifies MAPK14 as a druggable suppressor of human hematopoietic stem cell expansion.
Författare
Summary, in English
We report on a forward RNAi screen in primary human hematopoietic stem and progenitor cells, using pooled lentiviral shRNA libraries deconvoluted by next generation sequencing. We identify MAPK14/p38α as a modulator of ex vivo stem cell proliferation and show that pharmacological inhibition of p38 dramatically enhances the stem cell activity of cultured umbilical cord blood derived hematopoietic cells. p38 inhibitors should thus be considered in strategies aiming at expanding stem cells for clinical benefit.
Avdelning/ar
Publiceringsår
2012
Språk
Engelska
Sidor
6255-6258
Publikation/Tidskrift/Serie
Blood
Volym
119
Issue
26
Länkar
Dokumenttyp
Artikel i tidskrift
Förlag
American Society of Hematology
Ämne
- Hematology
Status
Published
ISBN/ISSN/Övrigt
- ISSN: 1528-0020