Isotope labeled internal standards (ILIS) as a basis for quality control in clinical studies using plasma samples
Författare
Summary, in English
For clinical proteomic studies the availability of high quality biofluid samples such as human blood plasma are extremely important. In this study we have investigated the stability of human plasma samples by spiking stable isotope-labeled peptides into the plasma and monitoring their degradation under different storage conditions. FPA-1, C4A and C3f were synthesized with isotopically labeled amino acids, and used as reference peptides. The mixture of internal calibrants was spiked into plasma at the starting point of investigation, mimicking the time of collection for future biobanking efforts, and their qualitative and quantitative changes were analyzed over time by using both MALDI-MS (LTQ Orbitrap XL) and nanoLC-ESI-MS (LTQ XL ETD).
We have found that all three synthetic peptides were stable in plasma at -20 and -80°C during the examined 2-month period. However, different proteolytic degradation profiles of the peptides were observed at room temperature. We anticipate that the use of these isotope-labeled peptides as internal standards (ILIS) provides a quality control for long-term storage and proteomic plasma analysis.
We have found that all three synthetic peptides were stable in plasma at -20 and -80°C during the examined 2-month period. However, different proteolytic degradation profiles of the peptides were observed at room temperature. We anticipate that the use of these isotope-labeled peptides as internal standards (ILIS) provides a quality control for long-term storage and proteomic plasma analysis.
Avdelning/ar
Publiceringsår
2010
Språk
Engelska
Sidor
1219-1229
Publikation/Tidskrift/Serie
Journal of Proteomics
Volym
73
Issue
6
Dokumenttyp
Artikel i tidskrift
Förlag
Elsevier
Ämne
- Medical Engineering
Nyckelord
- human blood plasma
- clinical proteomics
- mass spectrometry
- heavy isotope-labeled peptides
Status
Published
ISBN/ISSN/Övrigt
- ISSN: 1874-3919