A Probing Feeding Strategy for Escherichia coli Cultures
Författare
Summary, in English
A strain-independent feeding strategy for fed-batch cultures of <em>Escherichia coli</em> is presented. The key idea is to exploit a characteristic change in the cell metabolism at the onset of acetate formation. By superimposing short pulses in the glucose feed rate, on-line detection of acetate formation can be made using a standard dissolved oxygen sensor. A simple feedback algorithm is then used to adjust the feed rate to avoid acetate formation. The feasibility of the strategy is demonstrated by both simulation and experiments.
Avdelning/ar
Publiceringsår
1999
Språk
Engelska
Sidor
523-528
Publikation/Tidskrift/Serie
Biotechnology Techniques
Volym
13
Dokumenttyp
Artikel i tidskrift
Förlag
Springer
Ämne
- Control Engineering
Nyckelord
- Escherichia coli
- fed-batch culture
- glucose
- acetate
- feedback control
- dissolved oxygen.
Status
Published
ISBN/ISSN/Övrigt
- ISSN: 0951-208X