Biochemical Studies of Semenogelins with Emphasis on Zinc-Binding Properties
Författare
Summary, in English
The research findings reported in this thesis show that human SgI and SgII bind zinc(II) with a dissociation constant of 5 µM and a stoichiometry of at least ten ions per protein molecule. A truncated SgI lacking 60 amino acid residues, encoded by a polymorphic SgI-gene, was confirmed to have zinc(II)-binding capacity similar to that of the wild-type protein.
Circular dichroism spectroscopy revealed a heat stable secondary structure, and computer-based prediction of the spectra indicated that SgI (wild-type) contains 5?10% alpha-helices and 10?20% beta-sheets. The tertiary structure of SgI is changed upon binding of zinc(II), a property that may affect the ability of SgI to form a gel. Furthermore, it was discovered that the zinc(II)-binding properties of SgI enable direct measurements of the interaction between SgI and zinc(II) by surface plasmon resonance analysis.
Besides playing a structural role, zinc(II) binding to the semenogelins may have a regulatory function. It has been suggested that, by modulating the level of free zinc, the semenogelins control the activity of PSA (which is inhibited by zinc(II)). PSA can also be regulated by protein C inhibitor. Thus, a characterization of the binding between SgI and protein C inhibitor in the presence and absence of zinc(II) was carried out.
Avdelning/ar
Publiceringsår
2006
Språk
Engelska
Dokumenttyp
Doktorsavhandling
Förlag
Department of Laboratory Medicine, Lund University
Ämne
- Medicinal Chemistry
Nyckelord
- sexualitet
- sexuality
- Obstetrik
- gynekologi
- andrologi
- reproduction
- andrology
- gynaecology
- Obstetrics
- Klinisk kemi
- Clinical chemistry
- gel-forming proteins
- surface plasmon resonance
- prostate-specific antigen
- protein C inhibitor
- seminal plasma
- zinc
- semen
- semenogelin
- reproduktion
Status
Published
Forskningsgrupp
- Clinical Chemistry, Malmö
Handledare
ISBN/ISSN/Övrigt
- ISBN: 91-85481-82-3
Försvarsdatum
12 maj 2006
Försvarstid
13:15
Försvarsplats
Lilla aulan, MFC, Entrance 59, Malmö University Hospital, Malmö, Sweden
Opponent
- Per Venge (Professor)