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FTIR imaging and ATR-FT-Far-IR synchrotron spectroscopy of pig ear skin

In English

Författare

Summary, in English

FTIR imaging was performed on pig ear skin samples cryo-sectioned perpendicular to the skin surface. The OH-stretch region revealed the distribution of water; the amide II band gave the protein distribution; the C=O stretch and C-H stretch regions showed the variation in lipids. Water and proteins were similarly distributed. Triglycerides were predominantly found in the deeper skin layers whereas free fatty acids and ceramides were more dominant in the upper layers. ATR-FT-Far-IR spectroscopy with synchrotron radiation was used on full thickness pig ear skin biopsies. The Far-IR spectra showed hands in the region from 100-150 cm(-1) due to hydrogen bonded proteins and a band around 180 cm(-1) arising from "free" water.

Publiceringsår

2010

Språk

Engelska

Sidor

105-111

Publikation/Tidskrift/Serie

Spectroscopy: An International Journal

Volym

24

Issue

1-2

Dokumenttyp

Konferensbidrag

Förlag

IOS Press

Ämne

  • Physical Sciences
  • Natural Sciences

Nyckelord

  • pig
  • ear skin
  • synchrotron radiation
  • FTIR imaging
  • ATR-FT-Far-IR spectroscopy

Conference name

13th European Conference on Spectroscopy of Biological Mol

Conference date

2009-08-28 - 2009-09-02

Conference place

Palermo, Italy

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 0712-4813