Oligosaccharide mapping of proteoglycan-bound and xyloside-initiated dermatan sulfate from fibroblasts

Summary, in English

The copolymeric structure of dermatan sulfate chains synthesized by skin fibroblasts has been examined. Chains initiated onto exogeneous p-nitrophenyl-beta-D-xylopyranoside or attached to protein in a large proteoglycan, PG-L, and two small proteoglycans, PG-S1 and PG-S2, have been compared by using high resolution electrophoresis and gel chromatography of oligosaccharides generated by specific enzymatic or chemical degradations. The results confirm that chains attached to PG-L are glucuronate-rich, whereas novel findings indicate that chains attached to either of the two PG-S variants yield closely similar oligosaccharide maps, have approximately equal glucuronate and iduronate content and contain over 90% 4-sulfated disaccharide repeating units. Dermatan sulfate chains built onto xyloside at concentrations of 50 microM and below have a copolymeric structure similar to that of chains from the two PG-S variants. These findings indicate that the polymer-modifying machinery can generate chains with extended iduronate-containing repeats also when the xylose primer is not linked to core protein.

• Författare: Lars-Åke Fransson; Artur Schmidtchen; Lars Coster; Anders Malmström
• Avdelning/ar: Glykobiologigruppen; Dermatologi och venereologi, Lund; Matrix Biology
• Publiceringsår: 1991
• Språk: Engelska
• Sidor: 108-115
• Publikation/Tidskrift/Serie: Glycoconjugate Journal
• Volym: 8
• Issue: 2
• Länkar: http://dx.doi.org/10.1007/BF00731020
• Dokumenttyp: Artikel i tidskrift
• Förlag: Springer
• Ämne: Medicinal Chemistry; Cell and Molecular Biology; Dermatology and Venereal Diseases
• Nyckelord: oligosaccharide mapping; xylosides; dermatan sulfate; Proteoglycans
• Status: Published
• Forskningsgrupp: Glycobiology; Matrix Biology
• ISBN/ISSN/Övrigt: ISSN: 1573-4986