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Detection of Helicobacter species in chronic liver disease and chronic inflammatory bowel disease

In English

Författare

  • Lea Veijola
  • Ingrid Nilsson
  • Leena Halme
  • Waleed Abu Al-Soud
  • Judit Makinen
  • Åsa Ljungh
  • Hilpi Rautelin

Summary, in English

Aim. To study the association between helicobacters and chronic liver diseases and chronic inflammatory bowel diseases. Patients and methods. Thirty-two patients with various chronic liver diseases and 137 patients with inflammatory bowel disease were enrolled. Antibodies to H. pylori, H. hepaticus, H. bilis, and H. pullorum were measured by enzyme immunoassay (EIA), and sera positive in a non-pylori helicobacter EIA were further examined by immunoblot assay. Detection of Helicobacter DNA in liver biopsies was done by denaturating gradient gel electrophoresis of PCR products (PCR-DGGE) and DNA sequence analysis. Results. Six inflammatory bowel disease patients, four with ulcerative colitis and two with Crohn's disease, and one liver disease patient with autoimmune cholangitis had antibodies to non-pylori helicobacters by an immunoblot assay. Four immunoblot assay-negative patients, three with autoimmune and one with non-autoimmune liver disease, had Helicobacter DNA in liver biopsies; three of the polymerase chain reaction (PCR) products were closely related to non-pylori helicobacters. Conclusion. Evidence for non-pylori helicobacters was scant in Finnish patients with inflammatory bowel disease or chronic but not end stage liver disease. We cannot, however, rule out their role in these diseases.

Publiceringsår

2007

Språk

Engelska

Sidor

554-560

Publikation/Tidskrift/Serie

Annals of Medicine

Volym

39

Issue

7

Dokumenttyp

Artikel i tidskrift

Förlag

Taylor & Francis

Ämne

  • Microbiology in the medical area

Nyckelord

  • blotting
  • Western
  • PCR
  • liver disease
  • Helicobacter
  • inflammatory bowel disease

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 1365-2060