MSC from fetal and adult lungs possess lung-specific properties compared to bone marrow-derived MSC

Summary, in English

Mesenchymal stromal cells (MSC) are multipotent cells with regenerative and immune-modulatory properties. Therefore, MSC have been proposed as a potential cell-therapy for bronchiolitis obliterans syndrome (BOS). On the other hand, there are publications demonstrating that MSC might be involved in the development of BOS. Despite limited knowledge regarding the functional role of tissue-resident lung-MSC, several clinical trials have been performed using MSC, particularly bone marrow (BM)-derived MSC, for various lung diseases. We aimed to compare lung-MSC with the well-characterized BM-MSC. Furthermore, MSC isolated from lung-transplanted patients with BOS were compared to patients without BOS. Our study show that lung-MSCs are smaller, possess a higher colony-forming capacity and have a different cytokine profile compared to BM-MSC. Utilizing gene expression profiling, 89 genes including lung-specific FOXF1 and HOXB5 were found to be significantly different between BM-MSC and lung-MSC. No significant differences in cytokine secretion or gene expression were found between MSC isolated from BOS patients compared recipients without BOS. These data demonstrate that lung-resident MSC possess lung-specific properties. Furthermore, these results show that MSC isolated from lung-transplanted patients with BOS do not have an altered phenotype compared to MSC isolated from good outcome recipients.

• Författare: Sara Rolandsson Enes; Annika Andersson Sjöland; Ingrid Skog; Lennart Hansson; Hillevi Larsson; Katarina Le Blanc; Leif Eriksson; Leif Bjermer; Stefan Scheding; Gunilla Westergren-Thorsson
• Avdelning/ar: Lungbiologi; Lungmedicin, allergologi och palliativ medicin; Benmärgsstamceller och cellulära terapier; Stamcellscentrum (SCC); StemTherapy: National Initiative on Stem Cells for Regenerative Therapy; EpiHealth: Epidemiology for Health
• Publiceringsår: 2016-07-06
• Språk: Engelska
• Publikation/Tidskrift/Serie: Scientific Reports
• Volym: 6
• Länkar: http://dx.doi.org/10.1038/srep29160
• Dokumenttyp: Artikel i tidskrift
• Förlag: Nature Publishing Group
• Ämne: Basic Medicine
• Status: Published
• Forskningsgrupp: Lung Biology; Bone marrow stem cells and cellular therapies
• ISBN/ISSN/Övrigt: ISSN: 2045-2322