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Photopatterning of self assembled monolayers on oxide surfaces for the selective attachment of biomolecules

In English

Författare

  • Pompi Hazarika
  • Jonathan M. Behrendt
  • Linn Petersson
  • Christer Wingren
  • Michael L. Turner

Summary, in English

The immobilization of functional biomolecules to surfaces is a critical process for the development of biosensors for disease diagnostics. In this work we report the patterned attachment of single chain fragment variable (scFv) antibodies to the surface of metal oxides by the photodeprotection of self-assembled monolayers, using near-UV light. The photodeprotection step alters the functionality at the surface; revealing amino groups that are utilized to bind biomolecules in the exposed regions of the substrate only. The patterned antibodies are used for the detection of specific disease biomarker proteins in buffer and in complex samples such as human serum. (C) 2013 Elsevier B.V. All rights reserved.

Publiceringsår

2014

Språk

Engelska

Sidor

82-89

Publikation/Tidskrift/Serie

Biosensors & Bioelectronics

Volym

53

Dokumenttyp

Artikel i tidskrift

Förlag

Elsevier

Ämne

  • Immunology in the medical area

Nyckelord

  • Self-assembled monolayer (SAM)
  • Silicon
  • Photo-pattern
  • Proteins
  • Biomarker detection

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 1873-4235