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Colloidal Structures in Media Simulating Intestinal Fed State Conditions with and Without Lipolysis Products

In English

Författare

Summary, in English

Purpose. To study the ultrastructure of biorelevant media and digestion products of self-nanoemulsifying drug delivery system (SNEDDS) at high level BS/PL conditions. Methods. Cryogenic transmission electron microscopy (Cryo-TEM) was employed to visualize the colloid structures in the biorelevant media and lipolytic products generated during hydrolysis of a SNEDDS formulation. Their electrical properties were investigated by measuring their zeta-potential values. Results. In the biorelevant media, vesicles (either unilamellar or multilamellar) and bilayer fragments are visualized. Occasionally, vesicles with an internal deformed structure are recognized, suggesting surface tension or uneven lateral stress. Visualization studies of the intermediate colloidal phases produced during digestion of a SNEDDS using the in vitro lipolysis model revealed the formation of similar structures as previously reported. The zeta-potential of the media was negatively charged and decreased from -23 to -35 mV with increasing surfactant/lipid load. Lower zeta-potential values (-16 mV) obtained for the structures formed during the lipid hydrolysis of the SNEDDS were probably due to the presence of calcium, which shields the surface, thereby reducing the charge. Conclusions. The diversity of these vesicles in terms of size, lamellarity, and internal organization advocate their important role during lipid digestion in the gastrointestinal milieu.

Avdelning/ar

  • Department of Food Technology, Engineering and Nutrition

Publiceringsår

2009

Språk

Engelska

Sidor

361-374

Publikation/Tidskrift/Serie

Pharmaceutical Research

Volym

26

Issue

2

Dokumenttyp

Artikel i tidskrift

Förlag

Springer

Ämne

  • Pharmaceutical Sciences

Nyckelord

  • multilamellar vesicles
  • micelles
  • lipolytic products
  • in vitro digestion lipolysis model
  • microscopy
  • cryogenic transmission electron
  • zeta-potential
  • biorelevant media
  • unilamellar
  • systems
  • self-nanoemulsifying drug delivery

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 1573-904X