Polymerase chain reaction for detection of Mycobacterium tuberculosis

Summary, in English

A polymerase chain reaction for the specific detection of mycobacteria belonging to the Mycobacterium tuberculosis complex was developed. Using a single primer pair derived from the nucleotide sequence of protein antigen b of M. tuberculosis, we achieved specific amplification of a 419-base-pair DNA fragment in M. tuberculosis and M. bovis. After DNA was extracted from mycobacteria by using a simple, safe lysis procedure, we detected the 419-base-pair sequence in samples containing few mycobacteria. Preliminary data suggested that this technique could be applied to clinical specimens for early and specific diagnosis of tuberculosis.

• Författare: Ulf Sjöbring; Michael Mecklenburg; Åse Bengård Andersen; Håkan Miörner
• Avdelning/ar: Avdelningen för mikrobiologi, immunologi och glykobiologi - MIG; Avdelningen för medicinsk mikrobiologi
• Publiceringsår: 1990
• Språk: Engelska
• Sidor: 2200-2204
• Publikation/Tidskrift/Serie: Journal of Clinical Microbiology
• Volym: 28
• Issue: 10
• Länkar: http://jcm.asm.org/cgi/reprint/28/10/2200; http://www.pubmedcentral.nih.gov/picrender.fcgi?artid=268147&blobtype=pdf
• Dokumenttyp: Artikel i tidskrift
• Förlag: American Society for Microbiology
• Ämne: Microbiology in the medical area
• Status: Published
• ISBN/ISSN/Övrigt: ISSN: 1098-660X