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Actin-like protein associated with plasma membranes from Euglena gracilis

In English

Författare

Summary, in English

Microtubules are characteristic components of the membrane skeleton of Euglena gracilis, but whether microfilaments are present has been controversial. We here present evidence that an actin-like protein may indeed be associated with the plasma membrane (PM) of E. gracilis. Firstly, a 47 kDa, PM-associated, polypeptide was recognized by an anti-amoeba actin antibody. Secondly, this 47 kDa protein seemed to be peripherally attached to PM in much the same way as β-tubulin, since both could be released from PM by treatment with 150 mM NaOH but not with ethylene glycol, NaCl, or formamide. Thirdly, the 47 kDa polypeptide and β-tubulin were found mainly in the Triton X-1 14-insoluble fraction, indicating that they were part of a protein complex resistant to detergents, such as the cytoskeleton. Finally, DNase I activity was inhibited by a fraction enriched in the 47 kDa polypeptide, a property typical of actin.

Publiceringsår

1998

Språk

Engelska

Sidor

153-160

Publikation/Tidskrift/Serie

Protoplasma

Volym

202

Issue

3-4

Dokumenttyp

Artikel i tidskrift

Förlag

Springer

Ämne

  • Biological Sciences

Nyckelord

  • Actin
  • Anti-actin antibody
  • Cytoskeleton
  • Euglena gracilis
  • Triton X-114
  • DNAse I

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 1615-6102