Bladder volume alters cholinergic responses of the isolated whole mouse bladder
Författare
Summary, in English
Purpose: The isolated bladder expresses autonomous activity, which may contribute to the generation of lower urinary tract sensation or pathophysiology. We evaluated how the effect of a cholinergic agonist on autonomous activity alters with increasing volume and in the presence of substances known to modulate functional bladder capacity. Materials and Methods: The bladder of 22 adult female C57 black mice were mounted in whole organ tissue baths. Recordings of intravesical pressure were performed under standardized conditions at different bladder volumes. Results: At low volume the muscarinic agonist arecaidine elicited an initial peak response, which subsided to a sustained steady state pressure. At high volume phasic pressure fluctuations were also apparent. An M-2-receptor antagonist caused a significantly greater decrease in peak and steady state responses than in pressure fluctuations. An M-3-receptor antagonist decreased all 3 components. alpha, beta-methylene adenosine triphosphate markedly decreased fluctuations, in contrast to norepinephrine, which eliminated the steady state response while preserving fluctuations. Conclusions: The response to cholinergic stimulation of the isolated bladder has 3 components. The initial tonic peak response increases with bladder distention and it is inhibited by M-2 and M-3 muscarinic receptor antagonists. The tonic steady state response does not vary with bladder volume and it is inhibited by M-2 and M-3-receptor antagonists, and by beta(3)-adrenergic receptor agonists. Phasic fluctuations are minimal at low bladder volume, and with alpha, beta-methylene adenosine triphosphate or an M3-receptor antagonist. Thus, the response to cholinergic stimulation varies with bladder volume. It can be differentially modulated by muscarinic antagonists and also by agents acting through nonmuscarinic receptors.
Avdelning/ar
Publiceringsår
2006
Språk
Engelska
Sidor
771-776
Publikation/Tidskrift/Serie
Journal of Urology
Volym
175
Issue
2
Dokumenttyp
Artikel i tidskrift
Förlag
Lippincott Williams & Wilkins
Ämne
- Urology and Nephrology
Nyckelord
- muscle
- smooth
- mice
- bladder
- urinary incontinence
Status
Published
ISBN/ISSN/Övrigt
- ISSN: 1527-3792