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Dynamic gradients of an intermediate filament-like cytoskeleton are recruited by a polarity landmark during apical growth.

In English

Författare

  • Katsuya Fuchino
  • Sonchita Bagchi
  • Stuart Cantlay
  • Linda Sandblad
  • Di Wu
  • Jessica Bergman
  • Masood Kamali-Moghaddam
  • Klas Flärdh
  • Nora Ausmees

Summary, in English

Intermediate filament (IF)-like cytoskeleton emerges as a versatile tool for cellular organization in all kingdoms of life, underscoring the importance of mechanistically understanding its diverse manifestations. We showed previously that, in Streptomyces (a bacterium with a mycelial lifestyle similar to that of filamentous fungi, including extreme cell and growth polarity), the IF protein FilP confers rigidity to the hyphae by an unknown mechanism. Here, we provide a possible explanation for the IF-like function of FilP by demonstrating its ability to self-assemble into a cis-interconnected regular network in vitro and its localization into structures consistent with a cytoskeletal network in vivo. Furthermore, we reveal that a spatially restricted interaction between FilP and DivIVA, the main component of the Streptomyces polarisome complex, leads to formation of apical gradients of FilP in hyphae undergoing active tip extension. We propose that the coupling between the mechanism driving polar growth and the assembly of an IF cytoskeleton provides each new hypha with an additional stress-bearing structure at its tip, where the nascent cell wall is inevitably more flexible and compliant while it is being assembled and matured. Our data suggest that recruitment of cytoskeleton around a cell polarity landmark is a broadly conserved strategy in tip-growing cells.

Publiceringsår

2013

Språk

Engelska

Sidor

1889-1897

Publikation/Tidskrift/Serie

Proceedings of the National Academy of Sciences

Volym

110

Issue

21

Dokumenttyp

Artikel i tidskrift

Förlag

National Academy of Sciences

Ämne

  • Biological Sciences

Status

Published

Forskningsgrupp

  • Microbiology Group

ISBN/ISSN/Övrigt

  • ISSN: 1091-6490