Evaluating preservation medium for the storage of DNA in African lion Panthera leo faecal samples
Författare
Summary, in English
Lion faecal samples, collected in the field between 1 hour to 1 week after defecation were preserved in three different media (ethanol, ASL buffer and Two-step storage). The aim was to determine which faecal DNA field preservation method best enhances PCR amplification success. Samples stored in ethanol showed a significantly higher amplification success of microsatellite loci than samples stored in the other two media. In contrast, amplification success of a mitochondrial locus was similar among the samples stored in the three types of media. We reviewed twelve previous studies that employed different media for the storage of faeces, although patterns of success were not fully consistent among different media, ethanol storage was scored highest in the majority of these tests.
Avdelning/ar
Publiceringsår
2014
Språk
Engelska
Sidor
351-358
Publikation/Tidskrift/Serie
Current Zoology
Volym
60
Issue
3
Dokumenttyp
Artikel i tidskrift
Förlag
Oxford University Press
Ämne
- Biological Sciences
Nyckelord
- Amplification success
- Faecal sample DNA
- Lion Panthera leo
- PCR
- Preservation medium
Status
Published
Forskningsgrupp
- Molecular Ecology and Evolution Lab
ISBN/ISSN/Övrigt
- ISSN: 1674-5507