Multilayered Magnetic Nanoparticles as a Support in Solid-Phase Peptide Synthesis
Författare
Summary, in English
The synthesis of multilayered magnetic nanoparticles (MNPs) for use as a support in solid-phase peptide synthesis (SPPS) is described. Silanization of magnetite (Fe3O4) nanoparticles with 3-(trimethoxysilyl)propyl methacrylate introduced polymerizable groups on the surface. Polymerization with allylamine, trimethylolpropane trimethacrylate, and trimethylolpropane ethoxylate (14/3 EO/OH) triacrylate provided a polymeric coating and amino groups to serve as starting points for the synthesis. After coupling of an internal reference amino acid and a cleavable linker, the coated MNPs were applied as the solid phase during synthesis of Leu-enkephalinamide and acyl carrier protein (65-74) by Fmoc chemistry. A "high-load" version of the MNP support (0.32 mmol/g) was prepared by four consecutive cycles of Fmoc-Lys(Fmoc)-OH coupling and Fmoc deprotection. Successful synthesis of Leu-enkephalin was demonstrated on the "high-load" MNPs. Chemical stability studies proved the particles to be stable under SPPS conditions and magnetization measurements showed that the magnetic properties of the particles were maintained throughout derivatizations and SPPS. The MNPs were further characterized by high-resolution transmission electron microscopy, inductively coupled plasma atomic emission spectrometry, elemental analysis, and nitrogen gas adsorption measurements.
Avdelning/ar
Publiceringsår
2009
Språk
Engelska
Sidor
287-292
Publikation/Tidskrift/Serie
International Journal of Peptide Research and Therapeutics
Volym
15
Issue
4
Dokumenttyp
Artikel i tidskrift
Förlag
Springer
Ämne
- Pharmacology and Toxicology
Nyckelord
- Support
- Solid-phase peptide synthesis
- Polymer
- Multilayer structure
- Magnetite
- Magnetic materials
- Nanocomposites
Status
Published
Forskningsgrupp
- Nanomedicine and Biomaterials
ISBN/ISSN/Övrigt
- ISSN: 1573-3904