Webbläsaren som du använder stöds inte av denna webbplats. Alla versioner av Internet Explorer stöds inte längre, av oss eller Microsoft (läs mer här: * https://www.microsoft.com/en-us/microsoft-365/windows/end-of-ie-support).

Var god och använd en modern webbläsare för att ta del av denna webbplats, som t.ex. nyaste versioner av Edge, Chrome, Firefox eller Safari osv.

Transcriptional deregulation of myc in IgH/myc 6;7 translocation carrying rat immunocytomas

In English

Författare

  • H Axelson
  • W S Pear
  • C K Panda
  • H Bazin
  • G Klein
  • J Sümegi

Summary, in English

We have previously shown that the reciprocal translocation t(6;7) associated with the spontaneous immunocytoma of the Louvain rat (RIC) leads to the juxtaposition of myc to the IgH cluster. In 10 of 14 tumors investigated the breakpoints on the myc carrying chromosome were clustered in a 1.5 kb region 5' of the intact gene, proximal to the myc promoters. In this paper we describe the effect of the translocation on myc transcription in the RIC system. Run-on analysis showed transcriptional attenuation in the normal rat myc gene, similar to the situation in mice and humans. The attenuation was almost completely abrogated in the three immunocytomas studied. Sequence analysis of two tumors failed to reveal any structural changes within exon 1, as found by others in Burkitt's lymphoma. We also show that the transcriptional initiation of myc mRNA is changed in the RICs. In an established line of rat fibroblasts (Rat-2), the more distal myc promoter (P2) is the preferred site of initiation. In RIC, however, only 30% of transcripts were initiated from P2. We found that 40% of the transcripts were initiated from P1 and 30% from a novel promoter, designated P1a, located between P1 and P2.

Publiceringsår

1991-03

Språk

Engelska

Sidor

8-142

Publikation/Tidskrift/Serie

Genes, Chromosomes and Cancer

Volym

3

Issue

2

Dokumenttyp

Artikel i tidskrift

Förlag

John Wiley & Sons Inc.

Ämne

  • Medical Genetics

Nyckelord

  • Animals
  • Base Sequence
  • Burkitt Lymphoma
  • DNA Mutational Analysis
  • DNA, Neoplasm
  • Gene Expression Regulation, Neoplastic
  • Genes, Immunoglobulin
  • Genes, myc
  • Humans
  • Immunoglobulin Heavy Chains
  • Lymphoma
  • Mice
  • Molecular Sequence Data
  • Plasmacytoma
  • Promoter Regions, Genetic
  • Rats
  • Sequence Homology, Nucleic Acid
  • Species Specificity
  • Transcription, Genetic
  • Translocation, Genetic
  • Transplantation, Heterologous

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 1045-2257