Generating regionalized neuronal cells from pluripotency, a step-by-step protocol.
Författare
Summary, in English
Human pluripotent stem cells possess the potential to generate cells for regenerative therapies in patients with neurodegenerative diseases, and constitute an excellent cell source for studying human neural development and disease modeling. Protocols for neural differentiation of human pluripotent stem cells have undergone significant progress during recent years, allowing for rapid and synchronized neural conversion. Differentiation procedures can further be combined with accurate and efficient positional patterning to yield regionalized neural progenitors and subtype-specific neurons corresponding to different parts of the developing human brain. Here, we present a step-by-step protocol for neuralization and regionalization of human pluripotent cells for transplantation studies or in vitro analysis.
Avdelning/ar
Publiceringsår
2012
Språk
Engelska
Publikation/Tidskrift/Serie
Frontiers in Cellular Neuroscience
Volym
6
Issue
Published online: 03 January 2013
Fulltext
- Available as PDF - 421 kB
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Länkar
Dokumenttyp
Artikel i tidskrift
Förlag
Frontiers Media S. A.
Ämne
- Neurosciences
Status
Published
Forskningsgrupp
- Developmental and Regenerative Neurobiology
ISBN/ISSN/Övrigt
- ISSN: 1662-5102