The pTalpha promoter and enhancer are direct targets for transactivation by E box-binding proteins.
Författare
Summary, in English
The pre-Talpha (pTalpha[?]) gene encodes a protein that forms the pre-TCR together with the newly expressed TCR beta chain during a defined stage of early T cell development. The restricted expression of this gene has been suggested to depend on a promoter and an enhancer element containing E boxes, which are potential binding sites for basic helix-loop-helix (bHLH) proteins. We here report that increased expression of E47 and E12 mRNA in CD4(--)CD8(--)TCR(--) thymocytes correlates with the initiation of pTalpha[?] transcription. Furthermore, electrophoretic mobility shift assays suggested that E47 binds directly to both the pTalpha[?] promoter and enhancer in vitro. Ectopic expression of E47 in non-lymphoid HeLa cells resultedin a dose- and template-dependent activation of these control elements. In addition to this, ectopic expression of E47 in combination with the ets-protein Pu.1 and[?]/[?]or the Pu.1-interacting protein(Pip-1), resulted in a synergistic effect on the activity of the pTalpha[?] control elements. Thus, we suggest that E47, Pu.1 and Pip-1 are directly involved in the regulation of pTalpha expression inearly T cell development.
Avdelning/ar
- Cancerimmunologi, Malmö
- Immunology
- Stamcellscentrum (SCC)
Publiceringsår
2002
Språk
Engelska
Sidor
911-920
Publikation/Tidskrift/Serie
European Journal of Immunology
Volym
32
Issue
3
Länkar
Dokumenttyp
Artikel i tidskrift
Förlag
John Wiley & Sons Inc.
Ämne
- Immunology in the medical area
Nyckelord
- Gene Expression Regulation : genetics
- Hela Cells
- Genes T-Cell Receptor alpha
- Hamsters
- Enhancer Elements (Genetics)
- Electrophoretic Mobility Shift Assay
- DNA-Binding Proteins/biosynthesis : genetics : physiology
- Binding Sites
- Animal
- Promoter Regions (Genetics)
- Protein Binding
- Protein Precursors : genetics
- Proto-Oncogene Proteins : genetics : physiology
- RNA Messenger : genetics
- Recombinant Fusion Proteins : genetics : metabolism
- Regulatory Sequences Nucleic Acid
- Trans-Activation (Genetics)
- Trans-Activators : genetics : physiology
- Transfection
- Transcription Factors : physiology
- Helix-Loop-Helix Motifs
- Human
- Mesocricetus
- Mice
- Mice Inbred C57BL
Status
Published
Forskningsgrupp
- Cancer Immunology, Malmö
- Immunology
ISBN/ISSN/Övrigt
- ISSN: 1521-4141