Direct neural conversion from human fibroblasts using self-regulating and nonintegrating viral vectors.
Författare
Summary, in English
Recent findings show that human fibroblasts can be directly programmed into functional neurons without passing via a proliferative stem cell intermediate. These findings open up the possibility of generating subtype-specific neurons of human origin for therapeutic use from fetal cell, from patients themselves, or from matched donors. In this study, we present an improved system for direct neural conversion of human fibroblasts. The neural reprogramming genes are regulated by the neuron-specific microRNA, miR-124, such that each cell turns off expression of the reprogramming genes once the cell has reached a stable neuronal fate. The regulated system can be combined with integrase-deficient vectors, providing a nonintegrative and self-regulated conversion system that rids problems associated with the integration of viral transgenes into the host genome. These modifications make the system suitable for clinical use and therefore represent a major step forward in the development of induced neurons for cell therapy.
Avdelning/ar
Publiceringsår
2014
Språk
Engelska
Sidor
1673-1680
Publikation/Tidskrift/Serie
Cell Reports
Volym
9
Issue
5
Fulltext
Länkar
Dokumenttyp
Artikel i tidskrift
Förlag
Cell Press
Ämne
- Cell Biology
Status
Published
Forskningsgrupp
- Developmental and Regenerative Neurobiology
- Molecular Neurogenetics
ISBN/ISSN/Övrigt
- ISSN: 2211-1247