Gene expression profiling of differentiating embryonic stem cells expressing dominant negative fibroblast growth factor receptor 2.
Författare
Summary, in English
Embryonic stein (ES) cells are derived from the inner cell mass of the blastocyst and can be cultured as three-dimensional embryoid bodies (EBs) in which embryonic pregastrulation stages are faithfully mimicked. Fibroblast growth factor receptors (mainly FGFR2) are involved in the first differentiation events during early mammalian embryogenesis. It has been demonstrated that the presence of FGFR2 is a prerequisite for laminin-111 and collagen type IV synthesis and subsequently basement membrane formation in EBs. To identify genes that are influenced by FGFR signalling, we performed global gene expression profiling of differentiating EBs expressing dominant negative FGFR2 (dnFGFR2), acquiring an extensive catalogue of down- and up-regulated genes. We show a strong down-regulation of endodermal and basement membrane related genes, which strengthen the view that the FGFR signalling pathway is a main stimulator of basement membrane synthesis in EBs. We further present down-regulation of genes previously not linked to FGFR signalling, and in addition an active transcription of some mesodermal related genes in differentiating dnFGFR2 EBs.
Avdelning/ar
- Åke Oldberg´s group
- Muskelbiologi
- Institutionen för experimentell medicinsk vetenskap
Publiceringsår
2007
Språk
Engelska
Sidor
197-205
Publikation/Tidskrift/Serie
Matrix Biology
Volym
26
Fulltext
- Available as PDF - 345 kB
- Download statistics
Länkar
Dokumenttyp
Artikel i tidskrift
Förlag
Elsevier
Ämne
- Cell and Molecular Biology
Nyckelord
- ES cell differentiation
- Basement membrane
- Gene expression
- FGFR signalling
- Microarray
- Embryoid bodies
Status
Published
Forskningsgrupp
- Åke Oldberg´s group
- Muscle Biology
ISBN/ISSN/Övrigt
- ISSN: 1569-1802