Javascript verkar inte påslaget? - Vissa delar av Lunds universitets webbplats fungerar inte optimalt utan javascript, kontrollera din webbläsares inställningar.
Du är här

Microfluidic components for protein characterization.

Publiceringsår: 2001
Språk: Engelska
Sidor: 75-161
Publikation/Tidskrift/Serie: Journal of Biotechnology
Volym: 82
Nummer: 2
Dokumenttyp: Artikel i tidskrift
Förlag: Elsevier


The use of microfluidic components to create an analytical toolbox for the very rapidly growing field of proteomics is described. This toolbox provides novel generic analytical solutions that are highly adaptable for analysis of various biomolecules, ranging from high to low abundant. The components are fabricated using silicon micromachining and consist of a microchip immobilised enzyme reactor (microIMER), a piezoelectric microdispenser and high-density nanovial target plates. This microtechnology based platform interfaces matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI TOF-MS) to a wide range of upstream sample handling and/or analytical techniques. Examples of applications such as rapid on-line digestion (12 s) and sample preparation of proteins, interfacing to capillary liquid chromatography (100 attomol sensitivity), and in-vial chemistry on femtomol amounts of sample are presented.


  • Medical Engineering
  • Miniaturization
  • Nanotechnology
  • Proteins : chemistry
  • Proteins : isolation & purification
  • Proteome
  • Silicon
  • Spectrometry
  • Mass
  • Matrix-Assisted Laser Desorption-Ionization : instrumentation
  • Equipment Design
  • Biotechnology : instrumentation
  • Bioreactors


  • ISSN: 1873-4863

Box 117, 221 00 LUND
Telefon 046-222 00 00 (växel)
Telefax 046-222 47 20
lu [at] lu [dot] se

Fakturaadress: Box 188, 221 00 LUND
Organisationsnummer: 202100-3211
Om webbplatsen