Webbläsaren som du använder stöds inte av denna webbplats. Alla versioner av Internet Explorer stöds inte längre, av oss eller Microsoft (läs mer här: * https://www.microsoft.com/en-us/microsoft-365/windows/end-of-ie-support).

Var god och använd en modern webbläsare för att ta del av denna webbplats, som t.ex. nyaste versioner av Edge, Chrome, Firefox eller Safari osv.

Metabolism of [N-15]alanine in the ectomycorrhizal fungus Paxillus involutus

In English

Författare

Summary, in English

Alanine metabolism in the ectomycorrhizal fungus Paxillus involutus was investigated using [N-15]alanine. Short-term exposure of mycelial discs to [N-15]alanine showed that the greatest flow of N-15 was to glutamate and to aspartate. Levels of enrichment were as high as 15-20% for glutamate and 13-18% for aspartate, whereas that of alanine reached 30%. Label was also detected in the amino-N of glutamine and in serine and glycine, although at lower levels. Preincubation of mycelia with aminooxyacetate, an inhibitor of transamination reactions, resulted in complete inhibition of the flow of the label to glutamate, aspartate, and amino-N of glutamine, whereas [N-15]alanine rapidly accumulated. This evidence indicates the direct involvement of alanine aminotransferase for translocation of N-15 from alanine to glutamate. Alanine may be a convenient reservoir of both nitrogen and carbon. (C) 1995 Academic Press, Inc.

Publiceringsår

1995

Språk

Engelska

Sidor

297-304

Publikation/Tidskrift/Serie

Experimental Mycology

Volym

19

Issue

4

Dokumenttyp

Artikel i tidskrift

Förlag

Elsevier

Ämne

  • Biological Sciences

Nyckelord

  • alanine metabolism
  • alanine aminotransferase
  • aminooxyacetate
  • ectomycorrhizal fungus
  • organic nitrogen utilization
  • Paxillus involutus

Status

Published

Forskningsgrupp

  • Microbial Ecology

ISBN/ISSN/Övrigt

  • ISSN: 0147-5975