Webbläsaren som du använder stöds inte av denna webbplats. Alla versioner av Internet Explorer stöds inte längre, av oss eller Microsoft (läs mer här: * https://www.microsoft.com/en-us/microsoft-365/windows/end-of-ie-support).

Var god och använd en modern webbläsare för att ta del av denna webbplats, som t.ex. nyaste versioner av Edge, Chrome, Firefox eller Safari osv.

Characterization of specific IgE response in vitro against protein and drug allergens using atopic and normal donors

In English

Författare

  • A Åkesson
  • Sigurdur Ingvarsson
  • Fredrik Karlsson
  • L Leyva
  • M Blanca
  • SA Cuerden
  • JA Smith
  • JW Coleman
  • Carl Borrebaeck

Summary, in English

Background: As the incidence of allergy to different compounds increases in society, the need to understand and characterize specific IgE responses becomes F. obvious. Different cell culture systems have been evaluated for their ability to support such IgE secretion. Methods: One system employed human peripheral lymphocytes (PBL) from normal donors stimulated with anti-CD3 activated T cells with or without the presence of allergens like benzylpenicillin (BP) and Phlenum pratense (PP). Secretion of IgE was analyzed in ELISA and compared to the IgG response to the nonallergenic antigen tetanus toxoid (TT). Another system employed stimulation of T and B cells with a heterotope, consisting of a T helper cell epitope derived from TT, and a B cell allergen epitope derived from BP. The specific IgE secretion was compared, using lymphocytes from nor-Mal as well as BP-allergic donors. Results: Anti-CD3 stimulated T cells supported BP-specific IgE secretion in Six of 11 normal donors. This response was inhibited in four donors and enhanced in two donors by the addition of the BP-allergen to the culture. In contrast, addition of the protein allergen (PP) or antigen (TT) to the same culture system inhibited both IgE and IgG synthesis in all experiments. C-ells from the majority (10/16) of the BP-allergic donors failed to produce BP-specific IgE in vitro, when cultured in the presence of allergen. Conclusions: An allergen specific immune response is readily generated in vitro. The differential response against benzylpenicillin between different donor categories most probably reflects the level of pre-exposure to this allergen in vivo.

Publiceringsår

2002

Språk

Engelska

Sidor

193-200

Publikation/Tidskrift/Serie

Allergy

Volym

57

Issue

3

Dokumenttyp

Artikel i tidskrift

Förlag

Wiley-Blackwell

Ämne

  • Respiratory Medicine and Allergy

Nyckelord

  • in vitro
  • IgE
  • grasspollen
  • allergenicity
  • benzylpenicillin

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 1398-9995