Reduced hippocampal neurogenesis in R6/2 transgenic Huntington's disease mice.
Författare
Summary, in English
We investigated whether cell proliferation and neurogenesis are altered in R6/2 transgenic Huntington's disease mice. Using bromodeoxyuridine (BrdU), we found a progressive decrease in the number of proliferating cells in the dentate gyrus of R6/2 mice. This reduction was detected in pre-symptomatic mice, and by 11.5 weeks, R6/2 mice had 66% fewer newly born cells in the hippocampus. The results were confirmed by immunohistochemistry for the cell cycle markers Ki-67 and proliferating cell nuclear antigen (PCNA). We did not observe changes in cell proliferation in the R6/2 subventricular zone, indicating that the decrease in cell proliferation is specific for the hippocampus. This decrease corresponded to a reduction in actual hippocampal neurogenesis as assessed by double immunostaining for BrdU and the neuronal marker neuronal nuclei (NeuN) and by immunohistochemistry for the neuroblast marker doublecortin. Reduced hippocampal neurogenesis may be a novel neuropathological feature in R6/2 mice that could be assessed when evaluating potential therapies.
Avdelning/ar
- Institutionen för experimentell medicinsk vetenskap
- Wallenberg Neuroscience Centre, Lund
Publiceringsår
2005
Språk
Engelska
Sidor
744-751
Publikation/Tidskrift/Serie
Neurobiology of Disease
Volym
20
Issue
3
Länkar
Dokumenttyp
Artikel i tidskrift
Förlag
Elsevier
Ämne
- Neurosciences
Nyckelord
- Cell proliferation
- Dentate gyrus
- Doublecortin
- Huntington's disease
- Ki-67
- Neurogenesis
- R6/2 mice
- PCNA
- Subventricular zone
- BrdU
Status
Published
ISBN/ISSN/Övrigt
- ISSN: 0969-9961