Chiral Separation of Amino Acids by Ligand-Exchange Electrochromatography Using Continuous Beds
Författare
Summary, in English
A chiral ligand-exchange phase for capillary electrochromatography based on continu-ous bed technology was developed. The chiral stationary phase is prepared by a one- step in situ copolymerization procedure using methacrylamide, piperazine diacryla- mide, vinylsulfonic acid and N-(2-hydroxy-3-allyloxypropyl)-L-4-hydroxyproline. These chiral continuous beds are inexpensive and easy to prepare. They also have several advantages over silica-based packed capillaries. Since the bed is covalently attached to the capillary wall, no frit is required. The applicability of this new approach to the chi- ral separation of underivatized amino acids is demonstrated.
Publiceringsår
2000
Språk
Engelska
Sidor
3141-3144
Publikation/Tidskrift/Serie
Electrophoresis
Volym
21
Issue
15
Länkar
Dokumenttyp
Artikel i tidskrift
Förlag
John Wiley & Sons Inc.
Ämne
- Medical Engineering
Nyckelord
- Capillary electrochromatography
- Enantiomer separation
- Ligand-exchange
- Continuous beds
- Chiral beds
- Monolithic phases
Status
Published
ISBN/ISSN/Övrigt
- ISSN: 0173-0835