Directed antigen targeting in vivo identifies a role for CD103(+) dendritic cells in both tolerogenic and immunogenic T-cell responses.

Summary, in English

The αE integrin chain CD103 identifies a subset of migratory dendritic cells (DCs) in the gut, lung, and skin. To gain further understanding of the function of CD103(+) DCs in regulating adaptive immunity in vivo, we coupled ovalbumin (OVA) to the CD103 antibody M290 (M290.OVA). Intraperitoneal injection of M290.OVA induced OVA-specific CD8(+) and CD4(+) T-cell proliferation in lymph nodes (LNs) of wild-type but not CD103(-/-) mice, or in mice depleted of CD11c(+) cells. In the absence of maturation stimuli, systemic antigen targeting to CD103(+) DCs led to tolerance of CD8(+) T cells, whereas coadministration of adjuvant induced cytotoxic T-lymphocyte (CTL) immunity and antibody production. Mucosal intratracheal application of M290.OVA also induced T-cell proliferation in mediastinal LNs, yet the functional outcome was tolerance that inhibited subsequent development of allergic airway inflammation and immunoglobulin E (IgE) responses to inhaled OVA. These findings identify antigen targeting to CD103(+) DCs as a potential strategy to regulate immune responses in nonlymphoid mucosal tissues.Mucosal Immunology advance online publication 14 December 2011; doi:10.1038/mi.2011.61.

• Författare: Monika Semmrich; M Plantinga; Marcus Svensson Frej; Heli Uronen-Hansson; T Gustafsson; Allan Mowat; U Yrlid; B N Lambrecht; William Agace
• Avdelning/ar: Slemhinnans immunologi; Eosinophil Biology
• Publiceringsår: 2012
• Språk: Engelska
• Sidor: 150-160
• Publikation/Tidskrift/Serie: Mucosal Immunology
• Volym: 5
• Issue: 2
• Länkar: http://www.ncbi.nlm.nih.gov/pubmed/22166938?dopt=Abstract; http://dx.doi.org/10.1038/mi.2011.61
• Dokumenttyp: Artikel i tidskrift
• Förlag: Nature Publishing Group
• Ämne: Immunology in the medical area
• Status: Published
• Forskningsgrupp: Mucosal Immunology; Eosinophil Biology
• ISBN/ISSN/Övrigt: ISSN: 1933-0219