CCR2(+)CD103(-) intestinal dendritic cells develop from DC-committed precursors and induce interleukin-17 production by T cells.

Summary, in English

The identification of intestinal macrophages (mφs) and dendritic cells (DCs) is a matter of intense debate. Although CD103(+) mononuclear phagocytes (MPs) appear to be genuine DCs, the nature and origins of CD103(-) MPs remain controversial. We show here that intestinal CD103(-)CD11b(+) MPs can be separated clearly into DCs and mφs based on phenotype, gene profile, and kinetics. CD64(-)CD103(-)CD11b(+) MPs are classical DCs, being derived from Flt3 ligand-dependent, DC-committed precursors, not Ly6C(hi) monocytes. Surprisingly, a significant proportion of these CD103(-)CD11b(+) DCs express CCR2 and there is a selective decrease in CD103(-)CD11b(+) DCs in mice lacking this chemokine receptor. CCR2(+)CD103(-) DCs are present in both the murine and human intestine, drive interleukin (IL)-17a production by T cells in vitro, and show constitutive expression of IL-12/IL-23p40. These data highlight the heterogeneity of intestinal DCs and reveal a bona fide population of CCR2(+) DCs that is involved in priming mucosal T helper type 17 (Th17) responses.Mucosal Immunology advance online publication, 20 August 2014; doi:10.1038/mi.2014.70.

• Författare: C L Scott; C C Bain; P B Wright; D Sichien; Knut Kotarsky; Emma Persson; Katarzyna Luda; M Guilliams; B N Lambrecht; William Agace; S Wf Milling; Allan Mowat
• Avdelning/ar: Slemhinnans immunologi
• Publiceringsår: 2015
• Språk: Engelska
• Sidor: 327-339
• Publikation/Tidskrift/Serie: Mucosal Immunology
• Volym: 8
• Issue: 2
• Länkar: http://www.ncbi.nlm.nih.gov/pubmed/25138666?dopt=Abstract; http://dx.doi.org/10.1038/mi.2014.70
• Dokumenttyp: Artikel i tidskrift
• Förlag: Nature Publishing Group
• Ämne: Immunology in the medical area
• Status: Published
• Forskningsgrupp: Mucosal Immunology
• ISBN/ISSN/Övrigt: ISSN: 1933-0219