Isolation, characterization, and enzymatic hydrolysis of acetyl-galactoglucomannan from spruce (Picea abies)

Summary, in English

Water-soluble hemicelluloses were extracted from spruce chips by heat-fractionation using microwave treatment. A screening of conditions (pH, temperature and residence time) was performed for the extraction of O-acetyl-galactoglucomannan (AcGGM). The yield and the average molecular weight of the extracted mannan were analysed using HPLC, size-exclusion chromatography (SEC) and mass-spectrometry. The pH during heat fractionation influenced the yield and the structure of AcGGM. The highest yield (78%) of AcGGM (average molecular weight 3800) was achieved with heat-fractionation in water at 190degrees C for 5 minutes. With 0.025% NaOH, an average molecular weight of 9500 was obtained at a yield of 30%. AcGGM molecular weight standard molecules were prepared using SEC. The structure of the AcGGM was determined using H-1-NMR. The enzymatic hydrolysis of AcGGM was studied using beta-mannanase and alpha-galactosidase

• Författare: Henrik Stålbrand; Jon Lundqvist; Alexandra Andersson; Per Hägglund; Lars Anderson; Folke Tjerneld; A Jacobs A,; A Teleman; O Dahlman; M Palm; Guido Zacchi
• Avdelning/ar: Biokemi och Strukturbiologi; Avdelningen för kemiteknik
• Publiceringsår: 2004
• Språk: Engelska
• Sidor: 66-78
• Publikation/Tidskrift/Serie: Hemicelluloses. science and technology. (ACS symposium series ; 864 )
• Volym: 864
• Dokumenttyp: Konferensbidrag
• Förlag: The American Chemical Society (ACS)
• Ämne: Biological Sciences
• Nyckelord: MICROWAVE IRRADIATION; PINUS-SILVESTRIS L; DEGRADING ENZYMES; GLUCOMANNAN; LIGNOCELLULOSE; CHROMATOGRAPHY; PRETREATMENT; HEMICELLULOSES; BETA-MANNANASE; POSITIONS
• Status: Published
• ISBN/ISSN/Övrigt: ISBN: 0-8412-3842-1