Webbläsaren som du använder stöds inte av denna webbplats. Alla versioner av Internet Explorer stöds inte längre, av oss eller Microsoft (läs mer här: * https://www.microsoft.com/en-us/microsoft-365/windows/end-of-ie-support).

Var god och använd en modern webbläsare för att ta del av denna webbplats, som t.ex. nyaste versioner av Edge, Chrome, Firefox eller Safari osv.

Characterisation of a flavonoid ligand of the fungal protein Alt a 1

In English

Författare

  • Mariá Garrido-Arandia
  • Javier Silva-Navas
  • Carmen Ramírez-Castillejo
  • Nuria Cubells-Baeza
  • Cristina Gómez-Casado
  • Domingo Barber
  • Juan C. Pozo
  • Pablo G. Melendi
  • Luis F. Pacios
  • Araceli Diáz-Perales

Summary, in English

Spores of pathogenic fungi are virtually ubiquitous and cause human disease and severe losses in crops. The endophytic fungi Alternaria species produce host-selective phytotoxins. Alt a 1 is a strongly allergenic protein found in A. alternata that causes severe asthma. Despite the well-established pathogenicity of Alt a 1, the molecular mechanisms underlying its action and physiological function remain largely unknown. To gain insight into the role played by this protein in the pathogenicity of the fungus, we studied production of Alt a 1 and its activity in spores. We found that Alt a 1 accumulates inside spores and that its release with a ligand is pH-dependent, with optimum production in the 5.0-6.5 interval. The Alt a 1 ligand was identified as a methylated flavonoid that inhibits plant root growth and detoxifies reactive oxygen species. We also found that Alt a 1 changes its oligomerization state depending on the pH of the surrounding medium and that these changes facilitate the release of the ligand. Based on these results, we propose that release of Alt a 1 should be a pathogenic target in approaches used to block plant defenses and consequently to favor fungal entry into the plant.

Publiceringsår

2016-09-16

Språk

Engelska

Publikation/Tidskrift/Serie

Scientific Reports

Volym

6

Dokumenttyp

Artikel i tidskrift

Förlag

Nature Publishing Group

Ämne

  • Immunology in the medical area

Status

Published

Forskningsgrupp

  • Mucosal Immunology

ISBN/ISSN/Övrigt

  • ISSN: 2045-2322